The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Instrument settings are shown in Figure 3. The drawing shows a standard microplate of 15 mm height covered with a lid. Step 1. It measures the optical density (OD) of samples in a microplate or in a cuvette at aThe cells were then washed three times with PBS, and the fluorescent intensity of the cells was measured with a SpectraMax M5 microplate reader (Molecular Devices, USA) at an excitation wavelength of 488 nm and emission wavelength of 525 nm. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Most papers they cultured bacteria to OD600>0. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. This assay is based on the disappearance of hydrogen peroxide (H 2 O 2) at 240 nm in a medium containing 20 mM H 2 O 2, 0. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. 1. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. The reader uses two scanning monochromators to determine the optimal excitation and emission settings for the spectral characteristics of. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. 2. Table 1. SpectraMax® ABS. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. (Beijing, China) and a BCA protein assay kit from Beijing Solarbio Technology Co. 0. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Compact and tunable absorbance microplate readers DATA SHEET. 40266 Email: drugdiscoverytech@lifetech. Figure 1. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. 1. 72 MB. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s. Microplate Readers Include the M3, M4, M5, and M5. SpectraMax iD3, iD5, i3x, i3, M3, M4, M5, M5e, SpectraMax L, 6186 Luminescence FlexStation 3. 3 Version 7. The dual monochromator optics allow the widest. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Microplate Readers are modular and . HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. Linear, Orbital Slow, and Orbital Fast. Label-free detection. 0 out of 5 $ 24,695. With optimized reagents, option to operate the readers. When the user applies a set temperature of 37°C, the top is heated to 37. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. The absorbance values at 450 nm were determined by the SpectraMax M5 microplate reader. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Pick the best speed, sensitivity, and price for your needs. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. 00 USD. Ltd. Then, 150 μL of dimethyl sulfoxide was added to each well and shaken gently for 10 min. The system. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. SoftMax Pro. The cells were incubated with compounds at the indicated doses for 1 h. 2. Molecular Devices Spectramax M5 Microplate Reader. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader;. The CellTiter-Glo assay from Promega uses luciferase. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Design of genetic materials Sequence of β-gal siRNA was designed (5'-CA GUU GCG CAG CCU GAA UGU U3', 5'AA CAU UCA GGC UGC GCA ACU G-3') and nonsense siRNA ( Silencer ® negative control siRNA). The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. When a feature or capability applies to only certain readers, this exception is noted. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. We would like to show you a description here but the site won’t allow us. Sale price $18,499. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Fluorescence Microplate Reader Comparison Chart. FlexStation™ II. com. We specialize in the full suite of AKTA™ FPLC systems, Molecular Devices SpectraMax™ microplate readers, BioTek microplate washers, YMC (formerly LEWA) Process Technologies systems, ChromaCon Contichrom® instruments, and. -Wide range of applications -Five preferred detection modes -Maximum signal with lowest background The dual monochromator optics allow the widest range of. Fluorescence Microplate Readers. The SpectraMax M2 and M2e are versatile and reliable. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. SpectraMax® M5e. Softmax Pro GxP Software 6. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. Add to Cart. 04 to 3. IMAP FP Settings in SoftMax Pro M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. Maximum signal with lowest background. view more . Molecular Devices. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. Examples: “Press carefully to engage,” “ Do not. A new microplate luminometry for the toxicity bioassay of chemicals on firefly luciferase, was developed using the multifunctional microplate reader (SpectraMax M5) to measure the luminous intensity of luciferase. For example,it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. The assay window spanned four orders of magnitude. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. When a SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. M5 SpectraMax M5e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5e models. Five preferred detection modes. Recertification is recommended every two years. Category 2 and 3 microplate readers use gradient heating as well. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Designed to provide the simplicity, flexibility and power required for advanced data analysis. 2. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. The K d was calculated with GraphPad Prism software (GraphPad Software Inc. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. Introduction. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. Temperature variation ± 0. Small, unbound molecules rotate rapidly in. This. Self Service Available. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. Page 28 Chapter 5: Read Modes and Read Types 1. Qualify the luminescence performance of the SpectraMax . SpectraMax® M4. Italic and boldface type are used for emphasis. Add to Cart. The PBS solution (10. VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® Plus 384 Absorbance Microplate Reader; SpectraMax® L (software version 5. No accessories are needed and SoftMax® Pro Software. Preparation of the peritoneal macrophages The peritoneal macrophages were obtained from the mice after intraperitoneally (i. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. While they play an important role in generating data from a wide range of. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Wash Buffer Concentrate was diluted 10-fold. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. 2. 2. Microplate Reader 96 & 384 Thermo Multiskan Ascent w. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. Levels of immunoreactivity were normalized to. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. (Shanghai, China). User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Download PDF. reader in the visible range between 405–750 nm with NIST and NMI. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 1. Molecular Devices. free tracer decreases, resulting in. 1. Both readers feature an 8-channel read head for fast reads and. Lift up the lid over the chamber to insert or remove a cuvette. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112. PURPOSE. 00 USD. 0 out of 5 $ 17,999 $ 16,995. Fill a clean plate with water. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuide SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. SpectraMax iD5 Multi-Mode Microplate Reader. Filterless readers provide consistent results with steady temperature regulation. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. If you do not have an iLab account you must first make one: would like to show you a description here but the site won’t allow us. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The Spectr. Data are normalized to vehicle-treated. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Modethe introduction of the SPECTRAmax PLUS microplate spectrophotometer and its PathCheck feature, the ability to determine the pathlength in each well of a microplate and automatically normalize the absorbance value to a 1 cm pathlength. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Choose cables that meet the requirement described in this appendix. We would like to show you a description here but the site won’t allow us. 96-1536. For more sample throughput, the system canProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. SpectraMax M5 microplate reader (Molecular Devices, California, USA) with excitation at 450 nm and emission at 570 nm. 0 out of 5 $ 17,999 $ 16,995. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Microplate Readers include the M3, M4, M5, and M5e models. Trilevel commercial control reagents (StanbioThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. The cells were harvested on ice and. SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E. ) injection of 3 mLThe fluorescence intensity was detected by using a SpectraMax M5 microplate reader from Molecular Devices Co. SpectraMax Glo Steady-Luc Reporter Assay Kit. MOLECULAR DEVICES SpectraMax M5 also includes a wide selection of curve-fitting functions, allowing users to perform accurate and reliable quantification of biochemical reactions. Examples: “Press carefully to engage,” “ Do not. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. 7 cells were pretreated with SG (0, 25, 50, and 100 μg/mL). It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. AdThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. l. both cuvette-based and microplate reader applications on the same instrument. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. For the Live/Dead assay, they were treated. keeping with best practices, reader performance should. The. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. If you do not have an iLab account you must first make one: The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 1. Fill a clean plate with water. Microplate Readers include the M3, M4, M5, and M5e models. 303 unable to cal dark current. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. 58% for the detection module and 2. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi. Published Dec 16, 2020 | Updated Dec 16, 2020. Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. com. 0 out of. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. spr. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!The microplate readers in SpectraMax M5 series can also provide high-throughput capabilities with its automated microplate handling and storage systems. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. Do not touch the clear surfaces of the cuvette. To quantify the A1AT in rat serum, standard curves were drawn for nA1AT, rA1AT-WT and rA1AT mutants, respectively. Page 1 of 8 . The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 1. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. 0 2. com 13. SpectraMax Lmicroplate luminometer. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. TR-FRET assay principle. on Molecular Devices microplate readers: SpectraMax® iD5, i3x, Paradigm®, and M5 Multi-Mode Microplate Readers. 7°C and the bottom to 36. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Add to Cart. SpectraMax® M5 Multi-Mode Microplate Reader (cat. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Add to Cart. Or, with the reader specifications noted from the Instrument Description section of your reader's. 00 USD. The calculated LOQ values were 0. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Table of Contents. Access this by going to Help >. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. When a feature or capability applies to only certain readers, this exception is noted. The spectrophotometric. This is equivalent to having 511 built-in filters,The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. Other technologies such as imaging. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Urea-induced unfolding of rA1AT-WT and the Q9N mutant was measured using a SpectraMax. Comprehensive software and validation Comprehensive data analysis and GxP solutions SoftMax. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. The absorbance values at 450 and 570 nm were determined using the SpectraMax M5 microplate reader (Molecular Devices, Silicon Valley, CA, United States). SpectraMax M5 reader settings are shown. The SpectraMax® iD3 Multi Mode Microplate . Then, after H 2 O 2 was added, the fluorescence was measured for each time period at 488 nm (excitation) and 525 nm (emission) wavelengths on a SpectraMax M5 microplate reader. The dual monochromator optics allow the. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Plates were then shaken at room temperature for 10 min and the absorbance at 570/630 nm was measured by using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). edu) to be added as a trained user in iLab. Microplate Capacity. 7°C (gradient heating design). • SpectraMax Mini Multi-Mode Microplate Reader (AccuBlue HiRange only) • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode. These lab workhorses are used in drug discovery, research, bioassay validation, and. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. flexibility of monochromator-based systems. Use similar settings for other Molecular Devices readers with fluorescence detection mode. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. These robust readers have been placed in labs from Antarctica to. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. 2. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Standard features include a. Bioz Stars score: 86/100, based on 1 PubMed citations. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. M3-M5e_manual. Example: [Drawer]. 0 OD for earlier models. SpectraMax® M5. AquaMax® 4000. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The SpectraMax® ABS Plus Microplate Reader can run . To block unwanted residual excitation light and minimize background interference, a 570 nm. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Ltd. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. All associated StakMax® stackers. 7 1. Turn on the microplate reader. . Fluorescence intensity was detected by. The drawing shows a standard microplate of 15 mm height covered with a lid. Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. 312 gain calibration failed. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112. The guide also covers the software features, the specifications, and the accessories of the systems. Optical absorbance. The cuvette must be in place when you read the plate. Recommended Optics Wavelength (nm) Wavelength. The raw data from each individual experiment were normalized to vehicle-treated HK-2. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. Read one sample or up to 384 inThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Molecular Devices. Here the delta mP was 336, and Z’ factor was 0. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. e. Example: [Drawer]. 5. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. Fluorescence from the Alamar blue® assay was detected by SpectraMax M5 microplate reader (Molecular Device Inc. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. iD5 i3x M5 Paradigm Delta mP 69 85 92 88 Average StDev (mP) 1. Microplate Reader Shelf. FlexStation™ III. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Molecular Devices SpectraMax® series microplate readers. Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. 1** Version 7. The detection of lactate dehydrogenase releaseBriefly, algae were harvested, centrifuged, and re-suspended with an equal volume of methanol at the end of the experiment. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. Add to Cart. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader (Recent Model) with One Year Warranty (Svc Contract Available) Molecular Devices SpectraMax™ M5e Microplate Reader with 90 Day Depot Warranty (Svc Contract Available). Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Five. Shimadzu 2450 UV–Vis spectrophotometer and SpectraMax M5 microplate reader were used to perform the optical characterization and measurements. Learn how to set up protocols, customize reports, export results, and more with this PDF document. Published Dec 16, 2020 | Updated Dec 16, 2020. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance,. 1. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Molecular Devices Spectramax M5e Multi Mode Microplate Reader; Related products. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. The assay applies fluorescence polarization to quantify Fc-containing IgG. 1. 2. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. 6 0. IMAP assays are based on binding of. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The SoftMax® Touch. SpectraTest Validation Plates form Molecular Devices such as ABS1 absorbance, FL1 fluorescence & LM1 luminescence validation plates are the valuable tools for verifying that absorbance, fluorescence, and luminescence-capable microplate readers are operating correctly for GLP and GMP laboratories. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. The Perkin Elmer Wallac 1420 VICTOR2™ is a high performing microplate reader built for multi-tasking functions. SoftMax Pro Software has been integrated by many leading robotics andFor readers without a cuvette port, simply check the box next to ‘PathCheck’. 5°C at 37°C. The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. One vial of Thaw-and-Use TIGIT Effector Cells was thawed and resuspended in cell recovery. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. 73. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e).